![]() Edman degradation sequencing provided the full sequences which revealed a high degree of homology between the three components and the circulating Acknowledgments Three components, with molecular weights of 3651, 3661, and 4112 Da, were purified by preparative RP-HPLC. The fractionated venom also revealed the clear presence of minor components in the 3–4 kDa range (Fig. Whole venom was diluted to a concentration of 10 mg/mL with MilliQ water, centrifuged at 1500 g for 2 h in Centricon Results and discussionĪ preliminary LC–MS screening of the whole venom permitted us to detect molecular weight components from 2 to 20 kDa (data not shown). microleptidotus (inland taipan) from Goydnor’s Lagoon, South Australia, Australia Oxyuranus scutellatus canni (New Guinea taipan) from Merauke, West Papua, and Oxyuranus scutellatus scutellatus (coastal taipan) from Cairns, Queensland, Australia, and pooled to minimise the effects of individual variations. ![]() Thus, these naturally occurring isoforms provide a new platform for further investigation of structure–function relationships of natriuretic peptides. TNP-a and TNP-b were also unable to competitively inhibit the binding of TNP-c in endothelium-denuded aortae (GC-A) or endothelium-intact aortae (NPR-C). TNP-c was equipotent to ANP in specific GC-A assays or aortic ring assays whereas TNP-a and TNP-b were either inactive (GC-A over-expressing cells and endothelium-denuded aortic rings) or weakly active (endothelium-intact aortic rings). ![]() These molecules differ from ANP/BNP through replacement of invariant residues within the 17-membered ring structure and by inclusion of proline residues in the C-terminal tail. They were isolated by HPLC, characterised by mass spectrometry and Edman analysis, and consist of 35–39 amino acid residues. Three natriuretic-like peptides (TNP-a, TNP-b, and TNP-c) were isolated from the venom of Oxyuranus microlepidotus (inland taipan) and were also present in the venoms of Oxyuranus scutellatus canni (New Guinea taipan) and Oxyuranus scutellatus scutellatus (coastal taipan).
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